› 4yt4

X-ray diffraction
2.2Å resolution

Iron guanylylpyridinol (FeGP) cofactor-reconstituted HmdII from Methanocaldococcus jannaschii

Released:
DOI: 10.2210/pdb4yt4/pdb
PDB 4yt4 coloured by chain and viewed from the front.
PDB 4yt4 coloured by chain and viewed from the side.
PDB 4yt4 coloured by chain and viewed from the top.
Source organism: Methanocaldococcus jannaschii
Primary publication:
Towards a functional identification of catalytically inactive [Fe]-hydrogenase paralogs.
Fujishiro T, Ataka K, Ermler U, Shima S
FEBS J. (2015)
PMID: 26094576

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
H(2)-forming methylenetetrahydromethanopterin dehydrogenase-related protein MJ1338 Chain: A
Molecule details ›
Chain: A
Length: 375 amino acids
Theoretical weight: 40.66 KDa
Source organism: Methanocaldococcus jannaschii
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q58734 (Residues: 1-353; Coverage: 100%)
Gene name: MJ1338
Sequence domains: H2-forming N5,N10-methylene-tetrahydromethanopterin dehydrogenase
Structure domains:

Ligands and Environments

1 bound ligand:
Ligand FE9 1 x FE9
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SLS BEAMLINE X10SA
Spacegroup: P41212
Unit cell:
a: 59.81Å b: 59.81Å c: 183.43Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.187 0.184 0.226
Expression system: Escherichia coli BL21(DE3)

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Citations

1 review citation

Heterologous Hydrogenase Overproduction Systems for Biotechnology-An Overview.
Fan et al. (2020)

1 mention without citation

Towards a functional identification of catalytically inactive [Fe]-hydrogenase paralogs.
Fujishiro et al. (2015)