› 4ys9

X-ray diffraction
2Å resolution

Ataxin-3 Carboxy-Terminal Region - Crystal C1 (tetragonal)

Released:
DOI: 10.2210/pdb4ys9/pdb
PDB entry 4ys9 coloured by chain, front view.
PDB entry 4ys9 coloured by chain, side view.
PDB entry 4ys9 coloured by chain, top view.
Source organisms:
Primary publication:
The 2.2-Angstrom resolution crystal structure of the carboxy-terminal region of ataxin-3.
Zhemkov VA, Kulminskaya AA, Bezprozvanny IB, Kim M
FEBS Open Bio 6 168-78 (2016)
PMID: 27047745

Function and Biology Details

Reaction catalysed: 
Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-142649 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecules (2 distinct):
Ataxin-3; Maltose/maltodextrin-binding periplasmic protein Chain: B
Molecule details ›
Chain: B
Length: 441 amino acids
Theoretical weight: 48.58 KDa
Source organisms: Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P0AEY0 (Residues: 27-392; Coverage: 99%)
  • Canonical: P54252 (Residues: 278-329; Coverage: 14%)
Gene names: ATX3, ATXN3, ECs5017, MJD, MJD1, SCA3, Z5632, malE
Sequence domains:

Ligands and Environments

Carbohydrate polymer : NEW Components: GLC
Carbohydrate polymer
1 bound ligand:
Ligand ZN 4 x ZN
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 19-ID
Spacegroup: P41
Unit cell:
a: 59.67Å b: 59.67Å c: 135.165Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.208 0.207 0.223
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

3 review citations

Proteins Containing Expanded Polyglutamine Tracts and Neurodegenerative Disease.
Adegbuyiro et al. (2017)
2 more

1 mention without citation

The 2.2-Angstrom resolution crystal structure of the carboxy-terminal region of ataxin-3.
Zhemkov et al. (2016)