4yr8

X-ray diffraction
2.4Å resolution

Crystal structure of JNK in complex with a regulator protein

Released:

Function and Biology Details

Reactions catalysed:
ATP + a protein = ADP + a phosphoprotein
[a protein]-serine/threonine phosphate + H(2)O = [a protein]-serine/threonine + phosphate
Protein tyrosine phosphate + H(2)O = protein tyrosine + phosphate
Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Mitogen-activated protein kinase 8 Chains: A, C, E, F
Molecule details ›
Chains: A, C, E, F
Length: 371 amino acids
Theoretical weight: 42.83 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P45983 (Residues: 1-363; Coverage: 85%)
  • Best match: P45983-3 (Residues: 1-363)
Gene names: JNK1, MAPK8, PRKM8, SAPK1, SAPK1C
Sequence domains: Protein kinase domain
Structure domains:
Dual specificity protein phosphatase 16 Chains: B, D, G, H
Molecule details ›
Chains: B, D, G, H
Length: 167 amino acids
Theoretical weight: 18.92 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q9BY84 (Residues: 156-301; Coverage: 22%)
Gene names: DUSP16, KIAA1700, MKP7
Sequence domains: Dual specificity phosphatase, catalytic domain
Structure domains: Protein tyrosine phosphatase superfamily

Ligands and Environments

1 bound ligand:
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRF BEAMLINE BL17U
Spacegroup: P1
Unit cell:
a: 58.151Å b: 74.85Å c: 134.81Å
α: 76.94° β: 84.32° γ: 67.44°
R-values:
R R work R free
0.218 0.217 0.239
Expression system: Escherichia coli BL21(DE3)