X-ray diffraction
1.78Å resolution

Crystal structure of TrmD, a M1G37 tRNA Methyltransferase with SAM-competitive compounds

Entry authors: Elkins PA, Bonnette WG, Stuckey JA

Function and Biology Details

Reaction catalysed:
S-adenosyl-L-methionine + guanine(37) in tRNA = S-adenosyl-L-homocysteine + N(1)-methylguanine(37) in tRNA
Biological process:
Cellular component:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Entry contents:
1 distinct polypeptide molecule
tRNA (guanine-N(1)-)-methyltransferase Chain: A
Molecule details ›
Chain: A
Length: 254 amino acids
Theoretical weight: 28.38 KDa
Source organism: Haemophilus influenzae Rd KW20
Expression system: Escherichia coli BL21(DE3)
  • Canonical: P43912 (Residues: 1-246; Coverage: 100%)
Gene names: HI_0202, trmD
Sequence domains: tRNA (Guanine-1)-methyltransferase
Structure domains:

Ligands and Environments

1 bound ligand:
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 21-ID-G
Spacegroup: R32
Unit cell:
a: 94.39Å b: 94.39Å c: 178.917Å
α: 90° β: 90° γ: 120°
R R work R free
0.164 0.163 0.196
Expression system: Escherichia coli BL21(DE3)