X-ray diffraction
1.45Å resolution

Crystal structure of TrmD, a M1G37 tRNA Methyltransferase with SAM-competitive compounds

Entry authors: Elkins PA, Bonnette WG, Madauss KP

Function and Biology Details

Reaction catalysed:
S-adenosyl-L-methionine + guanine(37) in tRNA = S-adenosyl-L-homocysteine + N(1)-methylguanine(37) in tRNA
Biological process:
Cellular component:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Entry contents:
1 distinct polypeptide molecule
tRNA (guanine-N(1)-)-methyltransferase Chain: A
Molecule details ›
Chain: A
Length: 266 amino acids
Theoretical weight: 29.75 KDa
Source organism: Haemophilus influenzae Rd KW20
Expression system: Escherichia coli BL21(DE3)
  • Canonical: P43912 (Residues: 1-246; Coverage: 100%)
Gene names: HI_0202, trmD
Sequence domains: tRNA (Guanine-1)-methyltransferase
Structure domains:

Ligands and Environments

1 bound ligand:
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 5.0.2
Spacegroup: R32
Unit cell:
a: 94.376Å b: 94.376Å c: 177.996Å
α: 90° β: 90° γ: 120°
R R work R free
0.161 0.16 0.186
Expression system: Escherichia coli BL21(DE3)