X-ray diffraction
1.92Å resolution

DosS GAFA Domain Reduced CO Bound Crystal Structure


Function and Biology Details

Reaction catalysed:
ATP + protein L-histidine = ADP + protein N-phospho-L-histidine
Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Entry contents:
1 distinct polypeptide molecule
Oxygen sensor histidine kinase response regulator DevS/DosS Chains: A, B
Molecule details ›
Chains: A, B
Length: 154 amino acids
Theoretical weight: 16.84 KDa
Source organism: Mycobacterium tuberculosis CDC1551
Expression system: Escherichia coli BL21(DE3)
  • Canonical: P9WGK2 (Residues: 63-210; Coverage: 26%)
Gene names: MT3218, devS, dosS
Structure domains: Beta-Lactamase

Ligands and Environments

Cofactor: Ligand HEM 2 x HEM
1 bound ligand:
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 8.3.1
Spacegroup: P212121
Unit cell:
a: 33.505Å b: 79.247Å c: 109.451Å
α: 90° β: 90° γ: 90°
R R work R free
0.192 0.189 0.235
Expression system: Escherichia coli BL21(DE3)