Function and Biology Details
Reaction catalysed:
Hydrolysis of proteins with broad specificity.
Biochemical function:
Biological process:
Cellular component:
- not assigned
Sequence domains:
- Peptidase S8, subtilisin-related
- Peptidase S8/S53 domain superfamily
- Peptidase S8 propeptide/proteinase inhibitor I9
- Peptidase S8 propeptide/proteinase inhibitor I9 superfamily
- Subtilisin-like protease, fibronectin type-III domain
- Peptidase S8, subtilisin, Ser-active site
- Peptidase S8/S53 domain
- Subtilisin-like protease
1 more domain
Structure analysis Details
Assemblies composition:
Assembly name:
Cucumisin (preferred)
PDBe Complex ID:
PDB-CPX-174456 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (4 distinct):
Cucumisin
Molecule details ›
Chain: A
Length: 621 amino acids
Theoretical weight: 66.33 KDa
Source organism: Cucumis melo
UniProt:
Structure domains:
Length: 621 amino acids
Theoretical weight: 66.33 KDa
Source organism: Cucumis melo
UniProt:
- Canonical:
Q39547 (Residues: 111-731; Coverage: 88%)
Structure domains:
Cucumisin
Molecule details ›
Chain: B
Length: 96 amino acids
Theoretical weight: 10.66 KDa
Source organism: Cucumis melo
Expression system: Escherichia coli
UniProt:
Structure domains: Peptidase S8 propeptide/proteinase inhibitor I9
Length: 96 amino acids
Theoretical weight: 10.66 KDa
Source organism: Cucumis melo
Expression system: Escherichia coli
UniProt:
- Canonical: Q39547 (Residues: 23-110; Coverage: 12%)
Structure domains: Peptidase S8 propeptide/proteinase inhibitor I9
Ligands and Environments
No modified residues
Experiments and Validation Details
X-ray source:
SPRING-8 BEAMLINE BL32XU
Spacegroup:
P42
Expression system: Escherichia coli
