4ylm

X-ray diffraction
2.05Å resolution

Structure of PvcB, an Fe, alpha-ketoglutarate dependent oxygenase from an isonitrile synthetic pathway

Released:

Function and Biology Details

Reaction catalysed:
(2S)-3-(4-hydroxyphenyl)-2-isocyanopropanoate + 2-oxoglutarate + O(2) = (2E)-3-(4-hydroxyphenyl)-2-isocyanoprop-2-enoate + succinate + CO(2) + H(2)O
Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assemblies composition:
homo trimer (preferred)
homo hexamer
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Tyrosine isonitrile desaturase Chain: X
Molecule details ›
Chain: X
Length: 293 amino acids
Theoretical weight: 33.38 KDa
Source organism: Pseudomonas aeruginosa PAK
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q9I1L4 (Residues: 1-291; Coverage: 100%)
Gene names: PA2255, pvcB
Sequence domains: Taurine catabolism dioxygenase TauD, TfdA family
Structure domains: Clavaminate synthase-like

Ligands and Environments

1 bound ligand:
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 23-ID-B
Spacegroup: P6322
Unit cell:
a: 124.95Å b: 124.95Å c: 107.21Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.189 0.187 0.219
Expression system: Escherichia coli BL21(DE3)