› 4yjy

X-ray diffraction
1.86Å resolution

Crystal structure of Type III polyketide synthase from Oryza sativa

Released:
DOI: 10.2210/pdb4yjy/pdb
PDB entry 4yjy coloured by chain, front view.
PDB entry 4yjy coloured by chain, side view.
PDB entry 4yjy coloured by chain, top view.
Source organism: Oryza sativa Indica Group
Primary publication:
Synthetic Polyketide Enzymology: Platform for Biosynthesis of Antimicrobial Polyketides
Go MK, Wongsantichon J, Cheung VWN, Chow JY, Robinson RC, Yew WS
ACS Catalysis 5 4033-4042 (2015)

Function and Biology Details

Reaction catalysed: 
3 malonyl-CoA + 4-coumaroyl-CoA = 4 CoA + naringenin chalcone + 3 CO(2)
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-106990 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Chalcone synthase 1 Chains: A, B
Molecule details ›
Chains: A, B
Length: 398 amino acids
Theoretical weight: 43.29 KDa
Source organism: Oryza sativa Indica Group
Expression system: Escherichia coli
UniProt:
  • Canonical: A2ZEX7 (Residues: 1-398; Coverage: 100%)
Gene names: CHS, CHS1, OsI_035120
Sequence domains:
Structure domains: Peroxisomal Thiolase; Chain A, domain 1

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSRRC BEAMLINE BL13B1
Spacegroup: P212121
Unit cell:
a: 64.42Å b: 99.728Å c: 169.779Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.16 0.159 0.186
Expression system: Escherichia coli

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