4yj0

X-ray diffraction
3.81Å resolution

Crystal structure of the DM domain of human DMRT1 bound to 25mer target DNA

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero pentamer (preferred)
Entry contents:
1 distinct polypeptide molecule
2 distinct DNA molecules
Macromolecules (3 distinct):
Doublesex- and mab-3-related transcription factor 1 Chains: A, B, C
Molecule details ›
Chains: A, B, C
Length: 70 amino acids
Theoretical weight: 8.15 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q9Y5R6 (Residues: 70-131; Coverage: 17%)
Gene names: DMRT1, DMT1
Sequence domains: DM DNA binding domain
DNA (25-MER) Chain: D
Molecule details ›
Chain: D
Length: 25 nucleotides
Theoretical weight: 7.7 KDa
Source organism: Homo sapiens
Expression system: Not provided
DNA (25-MER) Chain: E
Molecule details ›
Chain: E
Length: 25 nucleotides
Theoretical weight: 7.65 KDa
Source organism: Homo sapiens
Expression system: Not provided

Ligands and Environments

1 bound ligand:
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 24-ID-C
Spacegroup: I222
Unit cell:
a: 83.185Å b: 138.926Å c: 141.574Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.232 0.229 0.262
Expression systems:
  • Escherichia coli BL21(DE3)
  • Not provided