› 4yin

X-ray diffraction
2.3Å resolution

Crystal structure of the extended-spectrum beta-lactamase OXA-145

Released:
DOI: 10.2210/pdb4yin/pdb
PDB entry 4yin coloured by chain, front view.
PDB entry 4yin coloured by chain, side view.
PDB entry 4yin coloured by chain, top view.
Source organism: Pseudomonas aeruginosa
Primary publication:
Structural insights into the loss of penicillinase and the gain of ceftazidimase activities by OXA-145 β-lactamase in Pseudomonas aeruginosa.
Meziane-Cherif D, Bonnet R, Haouz A, Courvalin P
J Antimicrob Chemother 71 395-402 (2016)
PMID: 26568564

Function and Biology Details

Reaction catalysed: 
A beta-lactam + H(2)O = a substituted beta-amino acid
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-111023 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Beta-lactamase Chains: A, B
Molecule details ›
Chains: A, B
Length: 246 amino acids
Theoretical weight: 27.36 KDa
Source organism: Pseudomonas aeruginosa
Expression system: Escherichia coli
UniProt:
  • Canonical: C0LZL5 (Residues: 20-265; Coverage: 100%)
Gene name: oxa145
Sequence domains: Penicillin binding protein transpeptidase domain
Structure domains: DD-peptidase/beta-lactamase superfamily

Ligands and Environments

1 bound ligand:
Ligand FLC 2 x FLC
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE BM30A
Spacegroup: P212121
Unit cell:
a: 46.725Å b: 91.182Å c: 125.284Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.192 0.189 0.248
Expression system: Escherichia coli

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Citations

1 review citation

A review on the mechanistic details of OXA enzymes of ESKAPE pathogens.
Avci et al. (2023)
4 other articles