4yhw

X-ray diffraction
3.25Å resolution

Yeast Prp3 (296-469) in complex with fragment of U4/U6 di-snRNA

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
PDBe Complex ID:
PDB-CPX-169878 (preferred)
Entry contents:
1 distinct polypeptide molecule
2 distinct RNA molecules
Macromolecules (3 distinct):
U4/U6 small nuclear ribonucleoprotein PRP3 Chains: A, B
Molecule details ›
Chains: A, B
Length: 177 amino acids
Theoretical weight: 21.37 KDa
Source organism: Saccharomyces cerevisiae
Expression system: Escherichia coli
UniProt:
  • Canonical: Q03338 (Residues: 296-469; Coverage: 37%)
Gene names: D8035.16, PRP3, RNA3, YDR473C
Sequence domains: Small nuclear ribonucleoprotein Prp3, C-terminal domain
U4 snRNA fragment Chains: C, D
Molecule details ›
Chains: C, D
Length: 17 nucleotides
Theoretical weight: 5.43 KDa
Source organism: Saccharomyces cerevisiae
Expression system: Not provided
U6 snRNA fragment Chains: E, F
Molecule details ›
Chains: E, F
Length: 27 nucleotides
Theoretical weight: 8.55 KDa
Source organism: Saccharomyces cerevisiae
Expression system: Not provided

Ligands and Environments

No bound ligands
1 modified residue:

Experiments and Validation Details

Entry percentile scores
X-ray source: BESSY BEAMLINE 14.2
Spacegroup: C2
Unit cell:
a: 144.672Å b: 59.565Å c: 109.783Å
α: 90° β: 118.52° γ: 90°
R-values:
R R work R free
0.251 0.248 0.299
Expression systems:
  • Escherichia coli
  • Not provided