4yhg

X-ray diffraction
2.4Å resolution

NATIVE BACTEROIDETES-AFFILIATED GH5 CELLULASE LINKED WITH A POLYSACCHARIDE UTILIZATION LOCUS

Released:

Function and Biology Details

Reaction catalysed:
Endohydrolysis of (1->4)-beta-D-glucosidic linkages in cellulose, lichenin and cereal beta-D-glucans
Biological process:
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
monomeric (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecules (2 distinct):
Cellulase domain-containing protein Chains: A, B
Molecule details ›
Chains: A, B
Length: 389 amino acids
Theoretical weight: 43.62 KDa
Source organism: Bacteroidetes bacterium AC2a
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: A0A076MPD7 (Residues: 26-414; Coverage: 99%)
Sequence domains: Cellulase (glycosyl hydrolase family 5)
Structure domains: Glycosidases

Ligands and Environments

Carbohydrate polymer : NEW Components: BGC
No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID29
Spacegroup: P212121
Unit cell:
a: 84.326Å b: 99.553Å c: 102.462Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.187 0.185 0.228
Expression system: Escherichia coli BL21(DE3)