› 4yfq

X-ray diffraction
1.62Å resolution

CRYSTAL STRUCTURE OF THE R111K:Y134F:T54V:R132Q:P39Y:R59Y MUTANT OF HUMAN CELLULAR RETINOIC ACID BINDING PROTEINII WITH RETINAL AFTER 24 HOURS INCUBATION AT 1.62 ANGSTROM RESOLUTION - Thermodynamic product - 1st cycle

Released:
DOI: 10.2210/pdb4yfq/pdb
PDB 4yfq coloured by chain and viewed from the front.
PDB 4yfq coloured by chain and viewed from the side.
PDB 4yfq coloured by chain and viewed from the top.
Source organism: Homo sapiens
Primary publication:
A Photoisomerizing Rhodopsin Mimic Observed at Atomic Resolution.
Nosrati M, Berbasova T, Vasileiou C, Borhan B, Geiger JH
J. Am. Chem. Soc. (2016)
PMID: 27310917

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assemblies composition:
monomeric
homo dimer (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Cellular retinoic acid-binding protein 2 Chain: A
Molecule details ›
Chain: A
Length: 137 amino acids
Theoretical weight: 15.58 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P29373 (Residues: 2-138; Coverage: 99%)
Gene name: CRABP2
Sequence domains: Lipocalin / cytosolic fatty-acid binding protein family
Structure domains: Lipocalin

Ligands and Environments

1 bound ligand:
Ligand RET 1 x RET
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 21-ID-F
Spacegroup: P3121
Unit cell:
a: 58.869Å b: 58.869Å c: 99.71Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.193 0.191 0.225
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

5 citation in other articles

Excited-state E → Z photoisomerization mechanism unveiled by ab initio nonadiabatic molecular dynamics simulation for hemithioindigo-hemistilbene.
Yang et al. (2017)
4 more

4 mentions without citation

A Photoisomerizing Rhodopsin Mimic Observed at Atomic Resolution.
Nosrati et al. (2016)
3 more