› 4yf5

X-ray diffraction
2Å resolution

Crystal structure of Rv1284 in the presence of polycarpine at acidic pH

Released:
DOI: 10.2210/pdb4yf5/pdb
PDB entry 4yf5 coloured by chain, front view.
PDB entry 4yf5 coloured by chain, side view.
PDB entry 4yf5 coloured by chain, top view.
Source organism: Mycobacterium tuberculosis CDC1551
Primary publication:
Chemical probing suggests redox-regulation of the carbonic anhydrase activity of mycobacterial Rv1284.
Nienaber L, Cave-Freeman E, Cross M, Mason L, Bailey UM, Amani P, A Davis R, Taylor P, Hofmann A
FEBS J 282 2708-21 (2015)
PMID: 25929542

Function and Biology Details

Reaction catalysed: 
H(2)CO(3) = CO(2) + H(2)O
Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-161987 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Beta-carbonic anhydrase 1 Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 172 amino acids
Theoretical weight: 19.26 KDa
Source organism: Mycobacterium tuberculosis CDC1551
Expression system: Escherichia coli 'BL21-Gold(DE3)pLysS AG'
UniProt:
  • Canonical: P9WPJ6 (Residues: 2-163; Coverage: 99%)
Gene names: MT1322, canA, mtcA1
Sequence domains: Carbonic anhydrase
Structure domains: Carbonic anhydrase

Ligands and Environments

2 bound ligands:
Ligand ZN 2 x ZN
Ligand CL 2 x CL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: AUSTRALIAN SYNCHROTRON BEAMLINE MX1
Spacegroup: F222
Unit cell:
a: 103.289Å b: 153.932Å c: 159.098Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.2 0.197 0.26
Expression system: Escherichia coli 'BL21-Gold(DE3)pLysS AG'

Quick links

Citations

2 review citations

Microbial Offense vs Host Defense: Who Controls the TB Granuloma?
Martinot AJ. (2018)
1 more

2 mentions without citation

Mycobacterium tuberculosisβ-Carbonic Anhydrases: Novel Targets for Developing Antituberculosis Drugs.
Aspatwar et al. (2019)
1 more