› 4yen

X-ray diffraction
2Å resolution

Room temperature X-ray diffraction studies of cisplatin binding to HEWL in DMSO media after 14 months of crystal storage - new refinement

Released:
DOI: 10.2210/pdb4yen/pdb
PDB entry 4yen coloured by chain, front view.
PDB entry 4yen coloured by chain, side view.
PDB entry 4yen coloured by chain, top view.
Source organism: Gallus gallus
Primary publication:
Crystallography and chemistry should always go together: a cautionary tale of protein complexes with cisplatin and carboplatin.
Shabalin I, Dauter Z, Jaskolski M, Minor W, Wlodawer A
Acta Crystallogr D Biol Crystallogr 71 1965-79 (2015)
PMID: 26327386

Function and Biology Details

Reaction catalysed: 
Hydrolysis of (1->4)-beta-linkages between N-acetylmuramic acid and N-acetyl-D-glucosamine residues in a peptidoglycan and between N-acetyl-D-glucosamine residues in chitodextrins
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-132831 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Lysozyme C Chain: A
Molecule details ›
Chain: A
Length: 129 amino acids
Theoretical weight: 14.33 KDa
Source organism: Gallus gallus
UniProt:
  • Canonical: P00698 (Residues: 19-147; Coverage: 100%)
Gene name: LYZ
Sequence domains: C-type lysozyme/alpha-lactalbumin family
Structure domains: Lysozyme

Ligands and Environments

3 bound ligands:
Ligand PT 2 x PT
Ligand CL 5 x CL
Ligand DMS 1 x DMS
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: BRUKER AXS MICROSTAR
Spacegroup: P43212
Unit cell:
a: 79.113Å b: 79.113Å c: 37.985Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.148 0.146 0.179

Quick links

Citations

4 review citations

A close look onto structural models and primary ligands of metallo-β-lactamases.
Raczynska et al. (2018)
3 more

2 mentions without citation

Crystallography and chemistry should always go together: a cautionary tale of protein complexes with cisplatin and carboplatin.
Shabalin et al. (2015)
1 more