› 4ydn

X-ray diffraction
1.35Å resolution

High resolution crystal structure of human transthyretin bound to ligand and conjugates of 4-(5-(3,5-dichloro-4-hydroxyphenyl)-1,3,4-oxadiazol-2-yl)phenyl fluorosulfate

Released:
DOI: 10.2210/pdb4ydn/pdb
PDB entry 4ydn coloured by chain, front view.
PDB entry 4ydn coloured by chain, side view.
PDB entry 4ydn coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
A fluorogenic aryl fluorosulfate for intraorganellar transthyretin imaging in living cells and in Caenorhabditis elegans.
Baranczak A, Liu Y, Connelly S, Du WG, Greiner ER, Genereux JC, Wiseman RL, Eisele YS, Bradbury NC, Dong J, Noodleman L, Sharpless KB, Wilson IA, Encalada SE, Kelly JW
J Am Chem Soc 137 7404-14 (2015)
PMID: 26051248

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-136168 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Transthyretin Chains: A, B
Molecule details ›
Chains: A, B
Length: 127 amino acids
Theoretical weight: 13.86 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P02766 (Residues: 21-147; Coverage: 100%)
Gene names: PALB, TTR
Sequence domains: HIUase/Transthyretin family
Structure domains: Transthyretin/hydroxyisourate hydrolase domain

Ligands and Environments

2 bound ligands:
Ligand 2WN 2 x 2WN
Ligand 4B8 1 x 4B8
1 modified residue:
Ligand 4AK 2 x 4AK

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRL BEAMLINE BL11-1
Spacegroup: P21212
Unit cell:
a: 43.093Å b: 85.522Å c: 64.65Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.159 0.157 0.195
Expression system: Escherichia coli

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Citations

7 review citations

Lysine-Targeted Inhibitors and Chemoproteomic Probes.
Cuesta et al. (2019)
6 more