PDBe 4yd1

X-ray diffraction
1.75Å resolution

Ternary complex of human DNA Polymerase Mu with 2-nt gapped DNA substrate and an incoming nonhydrolyzable dUMPNPP

Released:
Source organism: Homo sapiens
Primary publication:
Creative template-dependent synthesis by human polymerase mu.
Proc. Natl. Acad. Sci. U.S.A. (2015)
PMID: 26240373

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Entry contents:
1 distinct polypeptide molecule
3 distinct DNA molecules
Macromolecules (4 distinct):
DNA-directed DNA/RNA polymerase mu Chain: A
Molecule details ›
Chain: A
Length: 354 amino acids
Theoretical weight: 39.84 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9NP87 (Residues: 134-494; Coverage: 71%)
Gene names: POLM, polmu
Sequence domains:
Structure domains:
DNA (5'-D(*CP*GP*GP*CP*AP*AP*TP*AP*CP*G)-3') Chain: T
Molecule details ›
Chain: T
Length: 10 nucleotides
Theoretical weight: 3.05 KDa
Source organism: Homo sapiens
Expression system: Not provided
DNA (5'-D(*CP*GP*TP*A)-3') Chain: P
Molecule details ›
Chain: P
Length: 4 nucleotides
Theoretical weight: 1.19 KDa
Source organism: Homo sapiens
Expression system: Not provided
DNA (5'-D(P*GP*CP*CP*G)-3') Chain: D
Molecule details ›
Chain: D
Length: 4 nucleotides
Theoretical weight: 1.19 KDa
Source organism: Homo sapiens
Expression system: Not provided

Ligands and Environments


No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU MICROMAX-007 HF
Spacegroup: P212121
Unit cell:
a: 60.259Å b: 69.042Å c: 116.988Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.164 0.163 0.19
Expression systems:
  • Escherichia coli
  • Not provided