› 4ybg

X-ray diffraction
1.6Å resolution

Crystal structure of the MAEL domain of Drosophila melanogaster Maelstrom

Released:
DOI: 10.2210/pdb4ybg/pdb
PDB entry 4ybg coloured by chain, front view.
PDB entry 4ybg coloured by chain, side view.
PDB entry 4ybg coloured by chain, top view.
Source organism: Drosophila melanogaster
Primary publication:
Crystal Structure and Activity of the Endoribonuclease Domain of the piRNA Pathway Factor Maelstrom.
Matsumoto N, Sato K, Nishimasu H, Namba Y, Miyakubi K, Dohmae N, Ishitani R, Siomi H, Siomi MC, Nureki O
Cell Rep 11 366-75 (2015)
PMID: 25865890

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-194285 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Protein maelstrom Chain: A
Molecule details ›
Chain: A
Length: 255 amino acids
Theoretical weight: 29.11 KDa
Source organism: Drosophila melanogaster
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9VNS0 (Residues: 84-333; Coverage: 55%)
Gene names: CG11254, mael
Sequence domains: piRNA pathway germ-plasm component

Ligands and Environments

3 bound ligands:
Ligand ZN 1 x ZN
Ligand ACT 1 x ACT
Ligand EDO 1 x EDO
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SPRING-8 BEAMLINE BL32XU
Spacegroup: P43212
Unit cell:
a: 71.892Å b: 71.892Å c: 88.389Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.196 0.194 0.216
Expression system: Escherichia coli

Quick links

Citations

8 review citations

PIWI-interacting RNAs: small RNAs with big functions.
Ozata et al. (2019)
7 more

2 mentions without citation

Upgraded AMBER Force Field for Zinc-Binding Residues and Ligands for Predicting Structural Properties and Binding Affinities in Zinc-Proteins.
Macchiagodena et al. (2020)
1 more