› 4y5x

X-ray diffraction
3.15Å resolution

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero hexamer (preferred)
PDBe Complex ID:
PDB-CPX-210749 (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
diabody 310 VL domain Chains: A, D, G, J
Molecule details ›
Chains: A, D, G, J
Length: 136 amino acids
Theoretical weight: 14.22 KDa
Source organism: Homo sapiens
Expression system: Trichoplusia ni
Structure domains: Immunoglobulins
Ig-like domain-containing protein Chains: B, E, H, K
Molecule details ›
Chains: B, E, H, K
Length: 116 amino acids
Theoretical weight: 11.91 KDa
Source organism: Homo sapiens
Expression system: Trichoplusia ni
UniProt:
  • Canonical: Q5NV67 (Residues: 1-97; Coverage: 98%)
Gene name: V1-11
Sequence domains: Immunoglobulin V-set domain
Structure domains: Immunoglobulins
Erythropoietin receptor Chains: C, F, I, L
Molecule details ›
Chains: C, F, I, L
Length: 229 amino acids
Theoretical weight: 25.13 KDa
Source organism: Homo sapiens
Expression system: Trichoplusia ni
UniProt:
  • Canonical: P19235 (Residues: 32-249; Coverage: 45%)
Gene name: EPOR
Sequence domains:
Structure domains: Immunoglobulins

Ligands and Environments

2 bound ligands:
Ligand PEG 17 x PEG
Ligand FLC 1 x FLC
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRL BEAMLINE BL12-2
Spacegroup: P41212
Unit cell:
a: 239.54Å b: 239.54Å c: 132.569Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.204 0.201 0.241
Expression system: Trichoplusia ni

Quick links

Citations

26 review citations

Pathogenesis of myeloproliferative neoplasms.
Skoda et al. (2015)
25 more

2 mentions without citation

Crystal structures of mono- and bi-specific diabodies and reduction of their structural flexibility by introduction of disulfide bridges at the Fv interface.
Kim et al. (2016)
1 more