X-ray diffraction
2.1Å resolution

Thiazole synthase Thi4 from Methanococcus igneus


Function and Biology Details

Reaction catalysed:
NAD(+) + glycine + sulfide = nicotinamide + ADP-5-ethyl-4-methylthiazole-2-carboxylate + 3 H(2)O
Biochemical function:
Biological process:
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
homo octamer (preferred)
Entry contents:
1 distinct polypeptide molecule
Thiamine thiazole synthase Chains: A, B, C, D, E, F, G, H
Molecule details ›
Chains: A, B, C, D, E, F, G, H
Length: 286 amino acids
Theoretical weight: 30.84 KDa
Source organism: Methanotorris igneus Kol 5
Expression system: Escherichia coli
  • Canonical: F6BCS4 (Residues: 1-263; Coverage: 100%)
Gene names: Metig_0735, thi4
Sequence domains: Thi4 family
Structure domains: FAD/NAD(P)-binding domain

Ligands and Environments

2 bound ligands:
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 24-ID-C
Spacegroup: P31
Unit cell:
a: 101.302Å b: 101.302Å c: 204.309Å
α: 90° β: 90° γ: 120°
R R work R free
0.2 0.199 0.216
Expression system: Escherichia coli