› 4y2n

X-ray diffraction
2.4Å resolution

Structure of CFA/I pili major subunit CfaB trimer

Released:
DOI: 10.2210/pdb4y2n/pdb
PDB entry 4y2n coloured by chain, front view.
PDB entry 4y2n coloured by chain, side view.
PDB entry 4y2n coloured by chain, top view.
Source organism: Escherichia coli ETEC H10407
Primary publication:
Off-pathway assembly of fimbria subunits is prevented by chaperone CfaA of CFA/I fimbriae from enterotoxigenic E. coli.
Bao R, Liu Y, Savarino SJ, Xia D
Mol Microbiol 102 975-991 (2016)
PMID: 27627030

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
Sequence domain:

Structure analysis Details

Assembly composition:
homo hexamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-122893 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
CFA/I fimbrial subunit B Chains: A, B, C
Molecule details ›
Chains: A, B, C
Length: 153 amino acids
Theoretical weight: 15.94 KDa
Source organism: Escherichia coli ETEC H10407
Expression system: Escherichia coli
UniProt:
  • Canonical: E3PPC4 (Residues: 25-170; Coverage: 99%)
Gene names: ETEC_p948_0400, cfaB
Sequence domains: CS1 type fimbrial major subunit
Structure domains: CFA/I fimbrial subunit E, pilin domain

Ligands and Environments

2 bound ligands:
Ligand MG 3 x MG
Ligand EDO 1 x EDO
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 22-BM
Spacegroup: P321
Unit cell:
a: 114.723Å b: 114.723Å c: 67.081Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.24 0.237 0.26
Expression system: Escherichia coli

Quick links

Citations

2 citation in other articles

Enterotoxigenic E. coli virulence gene regulation in human infections.
Crofts et al. (2018)
1 more

1 mention without citation

Off-pathway assembly of fimbria subunits is prevented by chaperone CfaA of CFA/I fimbriae from enterotoxigenic E. coli.
Bao et al. (2016)