4y2c

X-ray diffraction
2.2Å resolution

Function and Biology Details

Reactions catalysed:
Nucleoside triphosphate + RNA(n) = diphosphate + RNA(n+1)
ATP + H(2)O = ADP + phosphate
Selective cleavage of Gln-|-Gly bond in the poliovirus polyprotein. In other picornavirus reactions Glu may be substituted for Gln, and Ser or Thr for Gly.
Biochemical function:
Biological process:
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
monomeric (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
RNA-directed RNA polymerase Chain: A
Molecule details ›
Chain: A
Length: 460 amino acids
Theoretical weight: 52.21 KDa
Source organism: Mengo virus
Expression system: Escherichia coli
UniProt:
  • Canonical: P12296 (Residues: 1834-2293; Coverage: 20%)
Sequence domains: Viral RNA-dependent RNA polymerase
Structure domains:

Ligands and Environments

1 bound ligand:
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ALBA BEAMLINE XALOC
Spacegroup: I4122
Unit cell:
a: 122.632Å b: 122.632Å c: 198.459Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.217 0.216 0.249
Expression system: Escherichia coli