› 4y1s

X-ray diffraction
1.61Å resolution

Structural basis for Ca2+-mediated interaction of the perforin C2 domain with lipid membranes

Released:
DOI: 10.2210/pdb4y1s/pdb
PDB entry 4y1s coloured by chain, front view.
PDB entry 4y1s coloured by chain, side view.
PDB entry 4y1s coloured by chain, top view.
Source organism: Mus musculus
Primary publication:
Structural Basis for Ca2+-mediated Interaction of the Perforin C2 Domain with Lipid Membranes.
Yagi H, Conroy PJ, Leung EW, Law RH, Trapani JA, Voskoboinik I, Whisstock JC, Norton RS
J Biol Chem 290 25213-26 (2015)
PMID: 26306037

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-145462 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Perforin-1 Chain: A
Molecule details ›
Chain: A
Length: 149 amino acids
Theoretical weight: 16.48 KDa
Source organism: Mus musculus
Expression system: Escherichia coli
UniProt:
  • Canonical: P10820 (Residues: 410-535; Coverage: 24%)
Gene names: Pfp, Prf1
Sequence domains: C2 domain
Structure domains: C2 domain

Ligands and Environments

1 bound ligand:
Ligand CA 1 x CA
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: AUSTRALIAN SYNCHROTRON BEAMLINE MX2
Spacegroup: P212121
Unit cell:
a: 37.7Å b: 43.211Å c: 68.077Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.167 0.166 0.185
Expression system: Escherichia coli

Quick links

Citations

4 review citations

Knocking 'em Dead: Pore-Forming Proteins in Immune Defense.
Liu et al. (2020)
3 more

2 mentions without citation

Structural Basis for Ca2+-mediated Interaction of the Perforin C2 Domain with Lipid Membranes.
Yagi et al. (2015)
1 more