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β-Lactoglobulin interactions with local anaesthetic drugs – Crystallographic and calorimetric studies.

Loch JI, Bonarek P, Polit A, Jabłoński M, Czub M, Ye X, Lewiński K
Int J Biol Macromol 80 87-94 (2015)
Related entries: 4y0p, 4y0q, 4y0s

Cited: 2 times
EuropePMC logo PMID: 26092174

Abstract

Interactions between bovine and goat β-lactoglobulin and tetracaine and pramocaine were investigated with isothermal titration calorimetry, X-ray crystallography and molecular modelling. Tetracaine and pramocaine binding to lactoglobulin is an entropy driven endothermic reaction. In this work, we found that determined association constants and thermodynamic parameters indicate that pramocaine has a higher affinity to lactoglobulin than tetracaine. Crystal structures that were determined with resolutions in the range from 1.90 to 2.30 Å revealed in each case the presence of a single drug molecule bound in the β-barrel in a mode similar to that observed for 14- and 16-carbon fatty acids. The position of the ligand in the β-barrel indicates the optimal fit of 6-carbon aromatic rings to the binding pocket and the major role of hydrophobic interactions in ligand binding. Calculations of tetracaine and pramocaine docking to lactoglobulin revealed that molecular modelling overestimated the role of polar protein-drug interactions.

Articles citing this publication (2)

  1. Molecular interaction of tea catechin with bovine β-lactoglobulin: A spectroscopic and in silico studies. Al-Shabib NA, Khan JM, Malik A, Tabish Rehman M, AlAjmi MF, Husain FM, Hisamuddin M, Altwaijry N. Saudi Pharm J 28 238-245 (2020)
  2. New ligand-binding sites identified in the crystal structures of β-lactoglobulin complexes with desipramine. Loch JI, Barciszewski J, Śliwiak J, Bonarek P, Wróbel P, Pokrywka K, Shabalin IG, Minor W, Jaskolski M, Lewiński K. IUCrJ 9 386-398 (2022)


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