4xzk

X-ray diffraction
1.8Å resolution

Crystal structure of ADP-ribosyltransferase Vis in complex with agmatine

Released:
Source organism: Vibrio splendidus
Entry authors: Pfoh R, Ravulapalli R, Merrill AR, Pai EF

Function and Biology Details

Reaction catalysed:
NAD(+) + protein-L-arginine = nicotinamide + N(omega)-(ADP-D-ribosyl)-protein-L-arginine
Sequence domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Putative NAD(+)--arginine ADP-ribosyltransferase Vis Chain: A
Molecule details ›
Chain: A
Length: 239 amino acids
Theoretical weight: 27.04 KDa
Source organism: Vibrio splendidus
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: A3UNN4 (Residues: 20-240; Coverage: 96%)
Gene name: V12B01_18061
Sequence domains: ADP-ribosyltransferase exoenzyme

Ligands and Environments

1 bound ligand:
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU MICROMAX-007 HF
Spacegroup: P212121
Unit cell:
a: 47.84Å b: 52.09Å c: 102.87Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.195 0.194 0.221
Expression system: Escherichia coli BL21(DE3)