› 4xzj

X-ray diffraction
1.8Å resolution

Crystal structure of ADP-ribosyltransferase Vis in complex with NAD

Released:
DOI: 10.2210/pdb4xzj/pdb
PDB entry 4xzj coloured by chain, front view.
PDB entry 4xzj coloured by chain, side view.
PDB entry 4xzj coloured by chain, top view.
Source organism: Vibrio splendidus
Primary publication:
Characterization of Vis Toxin, a Novel ADP-Ribosyltransferase from Vibrio splendidus.
Ravulapalli R, Lugo MR, Pfoh R, Visschedyk D, Poole A, Fieldhouse RJ, Pai EF, Merrill AR
Biochemistry 54 5920-36 (2015)
PMID: 26352925

Function and Biology Details

Reaction catalysed: 
NAD(+) + protein-L-arginine = nicotinamide + N(omega)-(ADP-D-ribosyl)-protein-L-arginine
Biochemical function:
Biological process:
Cellular component:
Sequence domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-107249 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Putative NAD(+)--arginine ADP-ribosyltransferase Vis Chain: A
Molecule details ›
Chain: A
Length: 239 amino acids
Theoretical weight: 27.04 KDa
Source organism: Vibrio splendidus
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: A3UNN4 (Residues: 20-240; Coverage: 96%)
Gene name: V12B01_18061
Sequence domains: ADP-ribosyltransferase exoenzyme

Ligands and Environments


Cofactor: Ligand NAD 1 x NAD
No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU MICROMAX-007 HF
Spacegroup: P212121
Unit cell:
a: 49.7Å b: 52Å c: 100.4Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.177 0.175 0.205
Expression system: Escherichia coli BL21(DE3)

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Citations

2 review citations

New Insights into Pathogenic Vibrios Affecting Bivalves in Hatcheries: Present and Future Prospects.
Dubert et al. (2017)
1 more

2 mentions without citation

14-3-3 proteins activate Pseudomonas exotoxins-S and -T by chaperoning a hydrophobic surface.
Karlberg et al. (2018)
1 more