› 4xye

X-ray diffraction
1.8Å resolution

GRANULICELLA M. FORMATE DEHYDROGENASE (FDH) IN COMPLEX WITH NAD(+)

Released:
DOI: 10.2210/pdb4xye/pdb
PDB 4xye coloured by chain and viewed from the front.
PDB 4xye coloured by chain and viewed from the side.
PDB 4xye coloured by chain and viewed from the top.
Source organism: Granulicella mallensis MP5ACTX8
Primary publication:
Structural basis for double cofactor specificity in a new formate dehydrogenase from the acidobacterium Granulicella mallensis MP5ACTX8.
Fogal S, Beneventi E, Cendron L, Bergantino E
Appl. Microbiol. Biotechnol. (2015)
PMID: 26104866

Function and Biology Details

Reaction catalysed: 
Formate + NAD(+) = CO(2) + NADH
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Formate dehydrogenase Chains: A, B
Molecule details ›
Chains: A, B
Length: 391 amino acids
Theoretical weight: 42.81 KDa
Source organism: Granulicella mallensis MP5ACTX8
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: G8NVB5 (Residues: 1-391; Coverage: 100%)
Gene name: AciX8_2172
Sequence domains:
Structure domains: NAD(P)-binding Rossmann-like Domain

Ligands and Environments

1 bound ligand:
Ligand NAD 2 x NAD
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID23-2
Spacegroup: P212121
Unit cell:
a: 59.05Å b: 113.847Å c: 115.319Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.153 0.151 0.187
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

7 citation in other articles

Discovery of an acidic, thermostable and highly NADP+ dependent formate dehydrogenase from Lactobacillus buchneri NRRL B-30929.
Alpdağtaş et al. (2018)
6 more

1 mention without citation

A Fifth of the Protein World: Rossmann-like Proteins as an Evolutionarily Successful Structural unit.
Medvedev et al. (2021)