› 4xwp

X-ray diffraction
1.82Å resolution

Structure of PE-PPE domains of ESX-1 secreted protein EspB, C2221 in presence of Ca

Released:
DOI: 10.2210/pdb4xwp/pdb
PDB entry 4xwp coloured by chain, front view.
PDB entry 4xwp coloured by chain, side view.
PDB entry 4xwp coloured by chain, top view.
Source organism: Mycobacterium tuberculosis H37Rv
Primary publication:
Structure of EspB, a secreted substrate of the ESX-1 secretion system of Mycobacterium tuberculosis.
Korotkova N, Piton J, Wagner JM, Boy-Röttger S, Japaridze A, Evans TJ, Cole ST, Pojer F, Korotkov KV
J Struct Biol 191 236-44 (2015)
PMID: 26051906

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-161685 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
ESX-1 secretion-associated protein EspB Chain: A
Molecule details ›
Chain: A
Length: 275 amino acids
Theoretical weight: 29.99 KDa
Source organism: Mycobacterium tuberculosis H37Rv
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P9WJD9 (Residues: 7-278; Coverage: 59%)
Gene names: MTV027.16c, Rv3881c, espB, mtb48
Sequence domains: ESX-1 secreted protein B PE domain

Ligands and Environments

1 bound ligand:
Ligand CA 1 x CA
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 22-ID
Spacegroup: C2221
Unit cell:
a: 66.24Å b: 69.51Å c: 119.14Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.201 0.199 0.234
Expression system: Escherichia coli BL21(DE3)

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Citations

7 review citations

ESX secretion systems: mycobacterial evolution to counter host immunity.
Gröschel et al. (2016)
6 more

1 mention without citation

Structure of EspB, a secreted substrate of the ESX-1 secretion system of Mycobacterium tuberculosis.
Korotkova et al. (2015)