PDBe 4xvc

X-ray diffraction
2Å resolution

Crystal structure of an esterase from the bacterial hormone-sensitive lipase (HSL) family

Released:

Function and Biology Details

Reaction catalysed:
A carboxylic ester + H(2)O = an alcohol + a carboxylate
Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
monomeric (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Esterase E40 Chains: A, B, C, D, E, F, G, H
Molecule details ›
Chains: A, B, C, D, E, F, G, H
Length: 317 amino acids
Theoretical weight: 34.3 KDa
Source organism: environmental samples
Expression system: Escherichia coli 'BL21-Gold(DE3)pLysS AG'
Structure domains: Rossmann fold

Ligands and Environments

1 bound ligand:

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRF BEAMLINE BL17U
Spacegroup: P1
Unit cell:
a: 74.63Å b: 76.172Å c: 130.186Å
α: 74.04° β: 75.15° γ: 72.6°
R-values:
R R work R free
0.198 0.196 0.232
Expression system: Escherichia coli 'BL21-Gold(DE3)pLysS AG'