4xv1

X-ray diffraction
2.47Å resolution

B-Raf Kinase V600E oncogenic mutant in complex with PLX7904

Released:

Function and Biology Details

Reaction catalysed:
ATP + a protein = ADP + a phosphoprotein
Biochemical function:
Biological process:
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Serine/threonine-protein kinase B-raf Chains: A, B
Molecule details ›
Chains: A, B
Length: 292 amino acids
Theoretical weight: 33.29 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P15056 (Residues: 444-705; Coverage: 34%)
Gene names: BRAF, BRAF1, RAFB1
Structure domains:

Ligands and Environments

1 bound ligand:

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 8.3.1
Spacegroup: P212121
Unit cell:
a: 51.331Å b: 104.817Å c: 110.228Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.236 0.234 0.273
Expression system: Escherichia coli