› 4xtm

X-ray diffraction
2.7Å resolution

Crystal structure of a recombinant Vatairea macrocarpa seed lectin complexed with GalNAc

Released:
DOI: 10.2210/pdb4xtm/pdb
PDB entry 4xtm coloured by chain, front view.
PDB entry 4xtm coloured by chain, side view.
PDB entry 4xtm coloured by chain, top view.
Source organism: Vatairea macrocarpa
Primary publication:
Crystal structure of a recombinant Vatairea macrocarpa seed lectin
Sousa BL, Silva-Filho JC, Kumar P, Lyskowski A, Bezerra GA, Delatorre P, Rocha BAM, Cunha RMS, Nagano CS, Gruber K, Cavada BS
Int. J. Biochem. Cell Biol. (2016)

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-160511 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Seed lectin Chain: A
Molecule details ›
Chain: A
Length: 250 amino acids
Theoretical weight: 27.5 KDa
Source organism: Vatairea macrocarpa
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: P81371 (Residues: 1-240; Coverage: 100%)
Sequence domains: Legume lectin domain
Structure domains: Jelly Rolls

Ligands and Environments

6 bound ligands:
Ligand CA 1 x CA
Ligand MN 1 x MN
Ligand A2G 1 x A2G
Ligand CIT 1 x CIT
Ligand GOL 1 x GOL
Ligand SO4 1 x SO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID29
Spacegroup: I222
Unit cell:
a: 56.354Å b: 86.111Å c: 129.023Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.207 0.205 0.244
Expression system: Escherichia coli BL21

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