4xt3

X-ray diffraction
3.8Å resolution

Structure of a viral GPCR bound to human chemokine CX3CL1

Released:
Source organisms:
Entry authors: Burg JS, Jude KM, Waghray D, Garcia KC

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (3 distinct):
G-protein coupled receptor homolog US28 Chain: A
Molecule details ›
Chain: A
Length: 362 amino acids
Theoretical weight: 42.04 KDa
Source organism: Cytomegalovirus
Expression system: Homo sapiens
UniProt:
  • Canonical: P69332 (Residues: 1-354; Coverage: 100%)
Gene name: US28
Sequence domains: 7 transmembrane receptor (rhodopsin family)
Structure domains: Rhodopsin 7-helix transmembrane proteins
Processed fractalkine Chain: B
Molecule details ›
Chain: B
Length: 91 amino acids
Theoretical weight: 10.01 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens
UniProt:
  • Canonical: P78423 (Residues: 25-101; Coverage: 21%)
Gene names: A-152E5.2, CX3CL1, FKN, NTT, SCYD1
Sequence domains: Small cytokines (intecrine/chemokine), interleukin-8 like
Structure domains: OB fold (Dihydrolipoamide Acetyltransferase, E2P)

Ligands and Environments

Carbohydrate polymer : NEW Components: NAG
2 bound ligands:
1 modified residue:

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 23-ID-D
Spacegroup: C2221
Unit cell:
a: 59.9Å b: 192.7Å c: 94.4Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.288 0.284 0.322
Expression system: Homo sapiens