4xq2

X-ray diffraction
2.1Å resolution

Ensemble refinement of cystathione gamma lyase (CalE6) D7G from Micromonospora echinospora

Released:
Source organism: Micromonospora echinospora
Entry authors: Wang F, Yennamalli RM, Singh S, Tan K, Thorson JS, Phillips Jr GN, Enzyme Discovery for Natural Product Biosynthesis (NatPro)

Function and Biology Details

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
CalE6 Chains: A, B, C, D, E, F, G, H
Molecule details ›
Chains: A, B, C, D, E, F, G, H
Length: 384 amino acids
Theoretical weight: 41.31 KDa
Source organism: Micromonospora echinospora
Expression system: Escherichia coli
UniProt:
  • Canonical: Q8KNG3 (Residues: 1-381; Coverage: 100%)
Gene name: calE6
Sequence domains: Cys/Met metabolism PLP-dependent enzyme
Structure domains:

Ligands and Environments

2 modified residues:

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 19-ID
Spacegroup: I222
Unit cell:
a: 146.855Å b: 146.981Å c: 349.905Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.145 0.143 0.183
Expression system: Escherichia coli