› 4xp8

X-ray diffraction
2.03Å resolution

Structure of EtgA D60N mutant

Released:
DOI: 10.2210/pdb4xp8/pdb
PDB 4xp8 coloured by chain and viewed from the front.
PDB 4xp8 coloured by chain and viewed from the side.
PDB 4xp8 coloured by chain and viewed from the top.
Source organism: Escherichia coli
Primary publication:
Structural analysis of a specialized type III secretion system peptidoglycan-cleaving enzyme.
Burkinshaw BJ, Deng W, Lameignère E, Wasney GA, Zhu H, Worrall LJ, Finlay BB, Strynadka NC
J. Biol. Chem. (2015)
PMID: 25678709

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
SLT domain-containing protein Chain: A
Molecule details ›
Chain: A
Length: 88 amino acids
Theoretical weight: 9.89 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
UniProt:
  • Canonical: C7BUG6 (Residues: 19-104; Coverage: 57%)
Gene name: etgA

Ligands and Environments

No bound ligands
1 modified residue:
Ligand MSE 2 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 8.2.1
Spacegroup: P3121
Unit cell:
a: 31.4Å b: 31.4Å c: 149.53Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.211 0.208 0.244
Expression system: Escherichia coli

Quick links

Citations

7 review citations

On the road to structure-based development of anti-virulence therapeutics targeting the type III secretion system injectisome.
Lyons et al. (2019)
6 more

1 mention without citation

Type Three Secretion System in Attaching and Effacing Pathogens.
Gaytán et al. (2016)