X-ray diffraction
1.9Å resolution

Crystal Structure of a GH7 Family Cellobiohydrolase from Daphnia pulex

Source organism: Daphnia pulex
Entry authors: Ebrahim A, Hobdey SE, Podkaminer K, Taylor II LE, Beckham GT, Decker SR, Himmel ME, Cragg SM, McGeehan JE

Function and Biology Details

Reaction catalysed:
Hydrolysis of (1->4)-beta-D-glucosidic linkages in cellulose and cellotetraose, releasing cellobiose from the non-reducing ends of the chains
Biological process:
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
monomeric (preferred)
Entry contents:
1 distinct polypeptide molecule
Cellobiohydrolase CHBI Chains: A, B
Molecule details ›
Chains: A, B
Length: 445 amino acids
Theoretical weight: 48.23 KDa
Source organism: Daphnia pulex
Expression system: Trichoderma reesei QM6a
  • Canonical: E9G5J5 (Residues: 20-464; Coverage: 100%)
Gene names: CEL7A, DAPPUDRAFT_347598
Sequence domains: Glycosyl hydrolase family 7
Structure domains: Glycoside hydrolase, family 7, domain

Ligands and Environments

1 bound ligand:
1 modified residue:

Experiments and Validation Details

Entry percentile scores
X-ray source: DIAMOND BEAMLINE I03
Spacegroup: C2
Unit cell:
a: 128.29Å b: 47.02Å c: 173.5Å
α: 90° β: 108.23° γ: 90°
R R work R free
0.166 0.164 0.21
Expression system: Trichoderma reesei QM6a