4xnf

X-ray diffraction
1.68Å resolution

Tailspike protein double mutant D339A/E372Q of E. coli bacteriophage HK620

Released:
Source organism: Salmonella virus HK620
Entry authors: Gohlke U, Broeker NK, Heinemann U, Seckler R, Barbirz S

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
homo trimer (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Head_binding domain-containing protein Chain: A
Molecule details ›
Chain: A
Length: 599 amino acids
Theoretical weight: 64.55 KDa
Source organism: Salmonella virus HK620
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: Q9AYY6 (Residues: 112-710; Coverage: 84%)
Gene name: 9
Sequence domains: Phage spike trimer
Structure domains:

Ligands and Environments

3 bound ligands:
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: BESSY BEAMLINE 14.1
Spacegroup: P321
Unit cell:
a: 74.222Å b: 74.222Å c: 174.337Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.156 0.154 0.196
Expression system: Escherichia coli BL21