4xmy

X-ray diffraction
1.45Å resolution

Tailspike protein double mutant D339A/E372A of E. coli bacteriophage HK620 in complex with pentasaccharide

Released:
Source organism: Enterobacteria phage HK620
Entry authors: Gohlke U, Broeker NK, Heinemann U, Seckler R, Barbirz S

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
homo trimer (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecules (2 distinct):
Head_binding domain-containing protein Chain: A
Molecule details ›
Chain: A
Length: 599 amino acids
Theoretical weight: 64.49 KDa
Source organism: Enterobacteria phage HK620
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q9AYY6 (Residues: 112-710; Coverage: 84%)
Gene name: 9
Sequence domains: Phage spike trimer
Structure domains:

Ligands and Environments

3 bound ligands:
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: BESSY BEAMLINE 14.1
Spacegroup: P321
Unit cell:
a: 74.234Å b: 74.234Å c: 174.578Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.146 0.144 0.174
Expression system: Escherichia coli BL21(DE3)