X-ray diffraction
2.7Å resolution

Anthrax toxin lethal factor with ligand-induced binding pocket


Function and Biology Details

Reaction catalysed:
Preferred amino acids around the cleavage site can be denoted BBBBxHx-|-H, in which B denotes Arg or Lys, H denotes a hydrophobic amino acid, and x is any amino acid. The only known protein substrates are mitogen-activated protein (MAP) kinase kinases.
Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
monomeric (preferred)
Entry contents:
1 distinct polypeptide molecule
Lethal factor Chain: A
Molecule details ›
Chain: A
Length: 519 amino acids
Theoretical weight: 60.44 KDa
Source organism: Bacillus anthracis
Expression system: Escherichia coli
  • Canonical: P15917 (Residues: 298-809; Coverage: 66%)
Gene names: BXA0172, GBAA_pXO1_0172, lef, pXO1-107
Sequence domains:
Structure domains:

Ligands and Environments

3 bound ligands:
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 17-ID
Spacegroup: P212121
Unit cell:
a: 57.028Å b: 78.423Å c: 139.269Å
α: 90° β: 90° γ: 90°
R R work R free
0.212 0.209 0.268
Expression system: Escherichia coli