Protein Data Bank in Europe

Succinyl-CoA + enzyme N(6)-(dihydrolipoyl)lysine = CoA + enzyme N(6)-(S-succinyldihydrolipoyl)lysine

D-galactose + O(2) = D-galacto-hexodialdose + H(2)O(2)

IMP + diphosphate = hypoxanthine + 5-phospho-alpha-D-ribose 1-diphosphate

dUTP + H(2)O = dUMP + diphosphate

Purine deoxynucleoside + phosphate = purine + 2'-deoxy-alpha-D-ribose 1-phosphate

1-(5-phospho-beta-D-ribosyl)-5-((5-phospho-beta-D-ribosylamino)methylideneamino)imidazole-4-carboxamide = 5-((5-phospho-1-deoxy-D-ribulos-1-ylamino)methylideneamino)-1-(5-phospho-beta-D-ribosyl)imidazole-4-carboxamide

2-phospho-4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol = 2-C-methyl-D-erythritol 2,4-cyclodiphosphate + CMP

5-phospho-beta-D-ribosylamine + diphosphate + L-glutamate = L-glutamine + 5-phospho-alpha-D-ribose 1-diphosphate + H(2)O

ATP + a protein = ADP + a phosphoprotein

N-carbamoylputrescine + H(2)O = putrescine + CO(2) + NH(3)

N-(5-phospho-D-ribosyl)-anthranilate + diphosphate = anthranilate + 5-phospho-alpha-D-ribose 1-diphosphate

S-adenosyl-L-methionine + pseudouridine(1915) in 23S rRNA = S-adenosyl-L-homocysteine + N(3)-methylpseudouridine(1915) in 23S rRNA

GTP = 3',5'-cyclic GMP + diphosphate

(1a) [acetyl-CoA C-acyltransferase]-S-acyl-L-cyteine + acetyl-CoA = 3-oxoacyl-CoA + [acetyl-CoA C-acyltransferase]-L-cyteine

ATP-dependent breakage, passage and rejoining of double-stranded DNA

(R)-propane-1,2-diol + NAD(+) = (R)-lactaldehyde + NADH

2 alpha,alpha'-trehalose 6-mycolate = alpha,alpha'-trehalose + alpha,alpha'-trehalose 6,6'-bismycolate

Release of an N-terminal amino acid, Xaa-|-Yaa-, in which Xaa is preferably Leu, but may be other amino acids including Pro although not Arg or Lys, and Yaa may be Pro. Amino acid amides and methyl esters are also readily hydrolyzed, but rates on arylamides are exceedingly low.

GTP + H(2)O = GDP + phosphate

4-amino-2-methyl-5-diphosphomethylpyrimidine + 2-((2R,5Z)-2-carboxy-4-methylthiazol-5(2H)-ylidene)ethyl phosphate = diphosphate + thiamine phosphate + CO(2)

2'-deoxyribonucleoside diphosphate + thioredoxin disulfide + H(2)O = ribonucleoside diphosphate + thioredoxin

Cleavage of peptide bonds with very broad specificity.

ATP-dependent cleavage of peptide bonds with broad specificity.

N(2)-acetyl-L-ornithine + L-glutamate = L-ornithine + N-acetyl-L-glutamate

2 6,7-dimethyl-8-(1-D-ribityl)lumazine = riboflavin + 4-(1-D-ribitylamino)-5-amino-2,6-dihydroxypyrimidine

Diphosphate + H(2)O = 2 phosphate

4 Fe(2+) + 4 H(+) + O(2) = 4 Fe(3+) + 2 H(2)O

3'-end directed exonucleolytic cleavage of viral RNA-DNA hybrid

ATP + H(2)O + 4 H(+)(Side 1) = ADP + phosphate + 4 H(+)(Side 2)

Chorismate = prephenate

L-asparagine + H(2)O = L-aspartate + NH(3)

Acyl-[acyl-carrier-protein] + malonyl-[acyl-carrier-protein] = 3-oxoacyl-[acyl-carrier-protein] + CO(2) + [acyl-carrier-protein]

2 L-tyrosyl-tRNA(Tyr) = 2 tRNA(Tyr) + cyclo(L-tyrosyl-L-tyrosyl)

L-alanine = D-alanine

D-fructose 1,6-bisphosphate = glycerone phosphate + D-glyceraldehyde 3-phosphate

Acetyl-CoA + [protein]-L-lysine = CoA + [protein]-N(6)-acetyl-L-lysine

Acetyl-CoA + alpha-D-glucosamine 1-phosphate = CoA + N-acetyl-alpha-D-glucosamine 1-phosphate

CoA-(4'-phosphopantetheine) + apo-[acyl-carrier-protein] = adenosine 3',5'-bisphosphate + holo-[acyl-carrier-protein]

Random endo-hydrolysis of N-acetyl-beta-D-glucosaminide (1->4)-beta-linkages in chitin and chitodextrins

Hydrolysis of (1->4)-beta-linkages between N-acetylmuramic acid and N-acetyl-D-glucosamine residues in a peptidoglycan and between N-acetyl-D-glucosamine residues in chitodextrins

Protein N(6)-(dihydrolipoyl)lysine + NAD(+) = protein N(6)-(lipoyl)lysine + NADH

Cyclo(L-tyrosyl-L-tyrosyl) + 2 reduced ferredoxin [iron-sulfur] cluster + 2 H(+) + O(2) = mycocyclosin + 2 oxidized ferredoxin [iron-sulfur] cluster + 2 H(2)O

Nitric oxide + H(2)O + ferricytochrome c = nitrite + ferrocytochrome c + 2 H(+)

ATP + shikimate = ADP + shikimate 3-phosphate

TSAVLQ-|-SGFRK-NH(2) and SGVTFQ-|-GKFKK the two peptides corresponding to the two self-cleavage sites of the SARS 3C-like proteinase are the two most reactive peptide substrates. The enzyme exhibits a strong preference for substrates containing Gln at P1 position and Leu at P2 position.

Tuberculosinyl diphosphate + adenosine = 1-tuberculosinyladenosine + diphosphate

Geranyl diphosphate + isopentenyl diphosphate = diphosphate + (2Z,6E)-farnesyl diphosphate

An acyl-[acyl-carrier protein] + NAD(+) = a trans-2,3-dehydroacyl-[acyl-carrier protein] + NADH

Isochorismate = salicylate + pyruvate

ATP + UDP-N-acetyl-alpha-D-muramoyl-L-alanyl-D-glutamate + meso-2,6-diaminoheptanedioate = ADP + phosphate + UDP-N-acetyl-alpha-D-muramoyl-L-alanyl-D-gamma-glutamyl-meso-2,6-diaminoheptanedioate

NTP + H(2)O = NDP + phosphate

dTDP-4-dehydro-6-deoxy-alpha-D-glucose = dTDP-4-dehydro-beta-L-rhamnose

Cleaves proteins of the adenovirus and its host cell at two consensus sites: -Yaa-Xaa-Gly-Gly-|-Xaa- and -Yaa-Xaa-Gly-Xaa-|-Gly- (in which Yaa is Met, Ile or Leu, and Xaa is any amino acid).

Deoxynucleoside triphosphate + DNA(n) = diphosphate + DNA(n+1)

ATP + pyruvate = ADP + phosphoenolpyruvate

5,10-methylenetetrahydrofolate + glycine + H(2)O = tetrahydrofolate + L-serine

UTP + N-acetyl-alpha-D-glucosamine 1-phosphate = diphosphate + UDP-N-acetyl-alpha-D-glucosamine

N-(5-phospho-beta-D-ribosyl)anthranilate = 1-(2-carboxyphenylamino)-1-deoxy-D-ribulose 5-phosphate

5,10-methylenetetrahydrofolate + dUMP + NADPH = dTMP + tetrahydrofolate + NADP(+)

Endohydrolysis of RNA in RNA/DNA hybrids. Three different cleavage modes: 1. sequence-specific internal cleavage of RNA. Human immunodeficiency virus type 1 and Moloney murine leukemia virus enzymes prefer to cleave the RNA strand one nucleotide away from the RNA-DNA junction. 2. RNA 5'-end directed cleavage 13-19 nucleotides from the RNA end. 3. DNA 3'-end directed cleavage 15-20 nucleotides away from the primer terminus.

5,10-methylenetetrahydrofolate + dUMP = dihydrofolate + dTMP

ATP = 3',5'-cyclic AMP + diphosphate

D-glyceraldehyde 3-phosphate = glycerone phosphate

Cleaves -Ala-|-Ser- and -Ala-|-Ala- bonds in the scaffold protein.

Hydrolysis of proteins to small peptides in the presence of ATP and magnesium. Alpha-Casein is the usual test substrate. In the absence of ATP, only oligopeptides shorter than five residues are hydrolyzed (such as succinyl-Leu-Tyr-|-NHMec; and Leu-Tyr-Leu-|-Tyr-Trp, in which cleavage of the -Tyr-|-Leu- and -Tyr-|-Trp bonds also occurs).

(1a) [enzyme]-L-histidine + 2,3-bisphospho-D-glycerate = [enzyme]-N(tau)-phospho-L-histidine + 2/3-phospho-D-glycerate

Selective hydrolysis of -Xaa-Xaa-|-Yaa- bonds in which each of the Xaa can be either Arg or Lys and Yaa can be either Ser or Ala.

Nucleoside triphosphate + RNA(n) = diphosphate + RNA(n+1)

Hydrolysis of four peptide bonds in the viral precursor polyprotein, commonly with Asp or Glu in the P6 position, Cys or Thr in P1 and Ser or Ala in P1'.

Trans,octacis-decaprenylphospho-beta-D-ribofuranose + FAD = trans,octacis-decaprenylphospho-beta-D-erythro-pentofuranosid-2-ulose + FADH(2)

L-lysine + 2-oxoglutarate = (S)-2-amino-6-oxohexanoate + L-glutamate

Androstan-3-alpha,17-beta-diol + NAD(+) = 17-beta-hydroxyandrostan-3-one + NADH

Peptidylproline (omega=180) = peptidylproline (omega=0)

5-methyltetrahydrofolate + NAD(P)(+) = 5,10-methylenetetrahydrofolate + NAD(P)H

S-adenosyl-L-methionine + a 5'-(N(7)-methyl 5'-triphosphoguanosine)-(ribonucleotide)-[mRNA] = S-adenosyl-L-homocysteine + a 5'-(N(7)-methyl 5'-triphosphoguanosine)-(2'-O-methyl-ribonucleotide)-[mRNA]

ATP + succinate + CoA = ADP + phosphate + succinyl-CoA

[a protein]-serine/threonine phosphate + H(2)O = [a protein]-serine/threonine + phosphate

Endohydrolysis of (1->4)-alpha-D-glucosidic linkages in polysaccharides containing three or more (1->4)-alpha-linked D-glucose units

D-fructose 1,6-bisphosphate + H(2)O = D-fructose 6-phosphate + phosphate

ATP + (R)-pantoate + beta-alanine = AMP + diphosphate + (R)-pantothenate

(S)-malate = fumarate + H(2)O

Succinate semialdehyde + NAD(P)(+) + H(2)O = succinate + NAD(P)H

L-quinate + NAD(P)(+) = 3-dehydroquinate + NAD(P)H

ADP-alpha-D-glucose + D-glucose 6-phosphate = ADP + alpha,alpha-trehalose 6-phosphate

ATP + thymidine = ADP + thymidine 5'-phosphate

Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).

Acetyl-CoA + H(2)O + glyoxylate = (S)-malate + CoA

Chorismate = isochorismate

ATP + protoporphyrin IX + Mg(2+) + H(2)O = ADP + phosphate + Mg-protoporphyrin IX + 2 H(+)

Hydrolyzes single-stranded DNA or mismatched double-stranded DNA and polynucleotides, releasing free uracil

Acyl-CoA + 1,2-diacylglycerol = CoA + triacylglycerol

S-formylglutathione + H(2)O = glutathione + formate

S-adenosyl-L-methionine + uracil(1498) in 16S rRNA = S-adenosyl-L-homocysteine + N(3)-methyluracil(1498) in 16S rRNA 

ATP + dTMP = ADP + dTDP

ATP + nucleoside diphosphate = ADP + nucleoside triphosphate

An ultra-long-chain mono-unsaturated acyl-[acyl-carrier protein] + a malonyl-[acyl-carrier protein] = an ultra-long-chain mono-unsaturated 3-oxo-fatty acyl-[acyl-carrier protein] + CO(2) + a holo-[acyl-carrier protein]

ATP + protein L-histidine = ADP + protein N-phospho-L-histidine

Nucleoside 3',5'-cyclic phosphate + H(2)O = nucleoside 5'-phosphate

Nucleoside 2',3'-cyclic phosphate + H(2)O = nucleoside 3'-phosphate

Beta-D-ribopyranose = beta-D-ribofuranose

ATP + glycerol = ADP + sn-glycerol 3-phosphate

Succinate + a quinone = fumarate + a quinol

Selective cleavage of Gln-|-Gly bond in the poliovirus polyprotein. In other picornavirus reactions Glu may be substituted for Gln, and Ser or Thr for Gly.

Selective cleavage of Tyr-|-Gly bond in picornavirus polyprotein.

4-(2-carboxyphenyl)-4-oxobutanoyl-CoA = 1,4-dihydroxy-2-naphthoyl-CoA + H(2)O

O-acetyl-L-serine + hydrogen sulfide = L-cysteine + acetate

Pyridoxamine 5'-phosphate + H(2)O + O(2) = pyridoxal 5'-phosphate + NH(3) + H(2)O(2)

Pyruvate + L-aspartate-4-semialdehyde = (4S)-4-hydroxy-2,3,4,5-tetrahydro-(2S)-dipicolinate + H(2)O

(3S)-3-hydroxyacyl-CoA = trans-2(or 3)-enoyl-CoA + H(2)O

AMP + H(2)O = D-ribose 5-phosphate + adenine

ATP + H(2)O + a folded polypeptide = ADP + phosphate + an unfolded polypeptide

Hydrolysis of terminal, non-reducing (1->4)-linked alpha-D-glucose residues with release of alpha-D-glucose

Hydrolysis of terminal, non-reducing beta-D-mannose residues in beta-D-mannosides

Acetyl-CoA + malonyl-[acyl-carrier-protein] = acetoacetyl-[acyl-carrier-protein] + CoA + CO(2) 

ATP + H(2)O = ADP + phosphate

5,6,7,8-tetrahydrofolate + NADP(+) = 7,8-dihydrofolate + NADPH

2-C-methyl-D-erythritol 4-phosphate + NADP(+) = 1-deoxy-D-xylulose 5-phosphate + NADPH

2 superoxide + 2 H(+) = O(2) + H(2)O(2)

Release of an N-terminal amino acid, preferentially leucine, but not glutamic or aspartic acids.

ATP + acetyl-CoA + HCO(3)(-) = ADP + phosphate + malonyl-CoA

ATP + propanoyl-CoA + HCO(3)(-) = ADP + phosphate + (S)-methylmalonyl-CoA