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STRUCTURE OF MUTANT (E165H) OF THE HSDR SUBUNIT OF THE ECOR124I RESTRICTION ENZYME IN COMPLEX WITH ATP

Source organism: Escherichia coli
Biochemical function: type I site-specific deoxyribonuclease activity
Biological process: DNA restriction-modification system
Cellular component: not assigned

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EC 3.1.21.3: Type I site-specific deoxyribonuclease

Reaction catalysed:
Endonucleolytic cleavage of DNA to give random double-stranded fragments with terminal 5'-phosphates, ATP is simultaneously hydrolyzed.
Systematic name:
-
Alternative Name(s):
  • ATP-dependent DNase
  • Adenosine triphosphate-dependent deoxyribonuclease
  • Deoxyribonuclease (ATP- and S-adenosyl-L-methionine-dependent)
  • Deoxyribonuclease (adenosine triphosphate-hydrolyzing)
  • Restriction-modification system
  • Type 1 site-specific deoxyribonuclease
  • Type I restriction enzyme

GO terms

Sequence families

Pfam Protein families (Pfam)
PF12008
Domain description: Type I restriction and modification enzyme - subunit R C terminal
Occurring in:
  1. Type I restriction enzyme EcoR124I/EcoR124II endonuclease subunit
The deposited structure of PDB entry 4xjx contains 2 copies of Pfam domain PF12008 (Type I restriction and modification enzyme - subunit R C terminal) in Type I restriction enzyme EcoR124I/EcoR124II endonuclease subunit. Showing 1 copy in chain B.

PF18766
Domain description: SWI2/SNF2 ATPase
Occurring in:
  1. Type I restriction enzyme EcoR124I/EcoR124II endonuclease subunit
The deposited structure of PDB entry 4xjx contains 2 copies of Pfam domain PF18766 (SWI2/SNF2 ATPase) in Type I restriction enzyme EcoR124I/EcoR124II endonuclease subunit. Showing 1 copy in chain B.

PF04313
Domain description: Type I restriction enzyme R protein N terminus (HSDR_N)
Occurring in:
  1. Type I restriction enzyme EcoR124I/EcoR124II endonuclease subunit
The deposited structure of PDB entry 4xjx contains 2 copies of Pfam domain PF04313 (Type I restriction enzyme R protein N terminus (HSDR_N)) in Type I restriction enzyme EcoR124I/EcoR124II endonuclease subunit. Showing 1 copy in chain B.

InterPro InterPro annotations
IPR027417
Domain description: P-loop containing nucleoside triphosphate hydrolase
Occurring in:
  1. Type I restriction enzyme EcoR124I/EcoR124II endonuclease subunit
IPR014001
Domain description: Helicase superfamily 1/2, ATP-binding domain
Occurring in:
  1. Type I restriction enzyme EcoR124I/EcoR124II endonuclease subunit
IPR022625
Domain description: Type I restriction enzyme R protein, C-terminal
Occurring in:
  1. Type I restriction enzyme EcoR124I/EcoR124II endonuclease subunit
IPR040980
Domain description: SWI2/SNF2 ATPase
Occurring in:
  1. Type I restriction enzyme EcoR124I/EcoR124II endonuclease subunit
IPR004473
Domain description: Restriction endonuclease, type I, HsdR
Occurring in:
  1. Type I restriction enzyme EcoR124I/EcoR124II endonuclease subunit
IPR007409
Domain description: Restriction endonuclease, type I, HsdR, N-terminal
Occurring in:
  1. Type I restriction enzyme EcoR124I/EcoR124II endonuclease subunit

Structure domains

CATH CATH domains
3.40.50.300
Class: Alpha Beta
Architecture: 3-Layer(aba) Sandwich
Topology: Rossmann fold
Homology: P-loop containing nucleotide triphosphate hydrolases
Occurring in:
  1. Type I restriction enzyme EcoR124I/EcoR124II endonuclease subunit
The deposited structure of PDB entry 4xjx contains 4 copies of CATH domain 3.40.50.300 (Rossmann fold) in Type I restriction enzyme EcoR124I/EcoR124II endonuclease subunit. Showing 2 copies in chain B.
3.90.1570.50
Class: Alpha Beta
Architecture: Alpha-Beta Complex
Topology: tt1808, chain A
Homology: tt1808, chain A
Occurring in:
  1. Type I restriction enzyme EcoR124I/EcoR124II endonuclease subunit
The deposited structure of PDB entry 4xjx contains 2 copies of CATH domain 3.90.1570.50 (tt1808, chain A) in Type I restriction enzyme EcoR124I/EcoR124II endonuclease subunit. Showing 1 copy in chain B.
3.90.640.50
Class: Alpha Beta
Architecture: Alpha-Beta Complex
Topology: Actin; Chain A, domain 4
Homology: Actin; Chain A, domain 4
Occurring in:
  1. Type I restriction enzyme EcoR124I/EcoR124II endonuclease subunit
The deposited structure of PDB entry 4xjx contains 2 copies of CATH domain 3.90.640.50 (Actin; Chain A, domain 4) in Type I restriction enzyme EcoR124I/EcoR124II endonuclease subunit. Showing 1 copy in chain B.

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