› 4xiu

X-ray diffraction
2.5Å resolution

Binary complex structure of Klenow fragment of Taq DNA polymerase I707L mutant with DNA containing TTT overhang

Released:
DOI: 10.2210/pdb4xiu/pdb
PDB entry 4xiu coloured by chain, front view.
PDB entry 4xiu coloured by chain, side view.
PDB entry 4xiu coloured by chain, top view.
Source organisms:
Primary publication:
A conservative isoleucine to leucine mutation causes major rearrangements and cold sensitivity in KlenTaq1 DNA polymerase.
Wu EY, Walsh AR, Materne EC, Hiltner EP, Zielinski B, Miller BR, Mawby L, Modeste E, Parish CA, Barnes WM, Kermekchiev MB
Biochemistry 54 881-9 (2015)
PMID: 25537790

Function and Biology Details

Reaction catalysed: 
Deoxynucleoside triphosphate + DNA(n) = diphosphate + DNA(n+1)
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-118264 (preferred)
Entry contents:
1 distinct polypeptide molecule
2 distinct DNA molecules
Macromolecules (3 distinct):
DNA polymerase I, thermostable Chain: A
Molecule details ›
Chain: A
Length: 539 amino acids
Theoretical weight: 60.87 KDa
Source organism: Thermus aquaticus
Expression system: Escherichia coli
UniProt:
  • Canonical: P19821 (Residues: 294-832; Coverage: 65%)
Gene names: pol1, polA
Sequence domains:
Structure domains:
Synthetic oligonucleotide primer strand Chain: B
Molecule details ›
Chain: B
Length: 12 nucleotides
Theoretical weight: 3.62 KDa
Source organism: synthetic construct
Expression system: Not provided
Synthetic oligonucleotide template strand Chain: C
Molecule details ›
Chain: C
Length: 14 nucleotides
Theoretical weight: 4.33 KDa
Source organism: synthetic construct
Expression system: Not provided

Ligands and Environments

1 bound ligand:
Ligand MG 1 x MG
1 modified residue:
Ligand DOC 1 x DOC

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 22-ID
Spacegroup: P3121
Unit cell:
a: 110.03Å b: 110.03Å c: 90.67Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.226 0.223 0.286
Expression systems:
  • Escherichia coli
  • Not provided

Quick links

Citations

2 review citations

Engineering Polymerases for New Functions.
Coulther et al. (2019)
1 more

2 mentions without citation

A conservative isoleucine to leucine mutation causes major rearrangements and cold sensitivity in KlenTaq1 DNA polymerase.
Wu et al. (2015)
1 more