4xig

X-ray diffraction
3.4Å resolution

Crystal structure of bacterial alginate ABC transporter determined through humid air and glue-coating method

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero pentamer (preferred)
Entry contents:
4 distinct polypeptide molecules
Macromolecules (5 distinct):
ABC transmembrane type-1 domain-containing protein Chain: M
Molecule details ›
Chain: M
Length: 301 amino acids
Theoretical weight: 34.29 KDa
Source organism: Sphingomonas sp.
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9KWT8 (Residues: 24-324; Coverage: 93%)
Gene name: algM1
Structure domains: MetI-like
ABC transmembrane type-1 domain-containing protein Chain: N
Molecule details ›
Chain: N
Length: 305 amino acids
Theoretical weight: 34.5 KDa
Source organism: Sphingomonas sp.
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9KWT7 (Residues: 1-293; Coverage: 100%)
Gene name: algM2
Structure domains: MetI-like
ABC transporter domain-containing protein Chains: S, T
Molecule details ›
Chains: S, T
Length: 363 amino acids
Theoretical weight: 39.57 KDa
Source organism: Sphingomonas sp. A1
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9KWT9 (Residues: 1-363; Coverage: 100%)
Gene name: algS
Sequence domains:
Structure domains:
AlgQ2 Chain: Q
Molecule details ›
Chain: Q
Length: 516 amino acids
Theoretical weight: 59.69 KDa
Source organism: Sphingomonas sp. A1
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9KWT5 (Residues: 1-516; Coverage: 100%)
Gene name: algQ2
Sequence domains: Bacterial extracellular solute-binding protein
Structure domains: Periplasmic binding protein-like II

Ligands and Environments

Carbohydrate polymer : NEW Components: BEM, MAW
No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SPRING-8 BEAMLINE BL38B1
Spacegroup: P212121
Unit cell:
a: 72.175Å b: 133.412Å c: 272.687Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.238 0.236 0.29
Expression system: Escherichia coli