› 4xha

X-ray diffraction
3Å resolution

Crystal structure of Thosea asigna virus RNA-dependent RNA polymerase (RdRP) complexed with Lu3+

Released:
DOI: 10.2210/pdb4xha/pdb
PDB entry 4xha coloured by chain, front view.
PDB entry 4xha coloured by chain, side view.
PDB entry 4xha coloured by chain, top view.
Source organism: Thosea asigna virus
Primary publication:
The Structure of the RNA-Dependent RNA Polymerase of a Permutotetravirus Suggests a Link between Primer-Dependent and Primer-Independent Polymerases.
Ferrero DS, Buxaderas M, Rodríguez JF, Verdaguer N
PLoS Pathog 11 e1005265 (2015)
PMID: 26625123

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domain:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-179389 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
RNA-dependent RNA polymerase Chains: A, B
Molecule details ›
Chains: A, B
Length: 705 amino acids
Theoretical weight: 79.54 KDa
Source organism: Thosea asigna virus
Expression system: Trichoplusia ni
UniProt:
  • Canonical: Q6A562 (Residues: 1-674; Coverage: 54%)
Gene name: RdRp

Ligands and Environments

3 bound ligands:
Ligand LU 2 x LU
Ligand SO4 8 x SO4
Ligand GOL 2 x GOL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID23-1
Spacegroup: C2221
Unit cell:
a: 154.05Å b: 224.57Å c: 128.29Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.185 0.182 0.244
Expression system: Trichoplusia ni

Quick links

Citations

6 review citations

RNA Dependent RNA Polymerases: Insights from Structure, Function and Evolution.
Venkataraman et al. (2018)
5 more

8 mentions without citation

Identification and characterization of novel nicotinic receptor-associated proteins in Caenorhabditis elegans.
Gottschalk et al. (2005)
7 more