PDBe 4xh9

X-ray diffraction
2Å resolution

CRYSTAL STRUCTURE OF HUMAN RHOA IN COMPLEX WITH DH/PH FRAGMENT OF THE GUANINE NUCLEOTIDE EXCHANGE FACTOR NET1

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Neuroepithelial cell-transforming gene 1 protein Chains: A, D
Molecule details ›
Chains: A, D
Length: 361 amino acids
Theoretical weight: 42.23 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli DH1
UniProt:
  • Canonical: Q7Z628 (Residues: 149-501; Coverage: 59%)
Gene names: ARHGEF8, NET1
Sequence domains:
Transforming protein RhoA Chains: B, E
Molecule details ›
Chains: B, E
Length: 180 amino acids
Theoretical weight: 20.29 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli DH1
UniProt:
  • Canonical: P61586 (Residues: 2-180; Coverage: 93%)
Gene names: ARH12, ARHA, RHO12, RHOA
Sequence domains: Ras family
Structure domains: P-loop containing nucleotide triphosphate hydrolases

Ligands and Environments

No bound ligands

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID23-1
Spacegroup: P21
Unit cell:
a: 54.1Å b: 101.4Å c: 116.2Å
α: 90° β: 94.3° γ: 90°
R-values:
R R work R free
0.19 0.188 0.217
Expression system: Escherichia coli DH1