4xgp

X-ray diffraction
1.9Å resolution

Crystal Structure of E112A/H234A Mutant of Stationary Phase Survival Protein (SurE) from Salmonella typhimurium co-crystallized and soaked with AMP.

Released:

Function and Biology Details

Reactions catalysed:
A 5'-ribonucleotide + H(2)O = a ribonucleoside + phosphate
A 3'-ribonucleotide + H(2)O = a ribonucleoside + phosphate
(Polyphosphate)(n) + H(2)O = (polyphosphate)(n-1) + phosphate
Biochemical function:
Biological process:
Cellular component:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
5'/3'-nucleotidase SurE Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 267 amino acids
Theoretical weight: 28.49 KDa
Source organism: Salmonella enterica subsp. enterica serovar Typhimurium str. LT2
Expression system: Escherichia coli
UniProt:
  • Canonical: P66881 (Residues: 1-253; Coverage: 100%)
Gene names: STM2927, surE
Sequence domains: Survival protein SurE
Structure domains: Survival protein SurE-like phosphatase/nucleotidase

Ligands and Environments

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE BM14
Spacegroup: C2
Unit cell:
a: 162.52Å b: 96.23Å c: 93.94Å
α: 90° β: 98.41° γ: 90°
R-values:
R R work R free
0.192 0.189 0.24
Expression system: Escherichia coli