› 4xgc

X-ray diffraction
3.5Å resolution

Crystal structure of the eukaryotic origin recognition complex

Released:
DOI: 10.2210/pdb4xgc/pdb
PDB entry 4xgc coloured by chain, front view.
PDB entry 4xgc coloured by chain, side view.
PDB entry 4xgc coloured by chain, top view.
Source organism: Drosophila melanogaster
Primary publication:
Crystal structure of the eukaryotic origin recognition complex.
Bleichert F, Botchan MR, Berger JM
Nature 519 321-6 (2015)
PMID: 25762138

Function and Biology Details

Reaction catalysed: 
NTP + H(2)O = NDP + phosphate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero heptamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-127567 (preferred)
Entry contents:
7 distinct polypeptide molecules
Macromolecules (7 distinct):
Origin recognition complex subunit 2 Chain: B
Molecule details ›
Chain: B
Length: 354 amino acids
Theoretical weight: 40.11 KDa
Source organism: Drosophila melanogaster
Expression system: Trichoplusia ni
UniProt:
  • Canonical: Q24168 (Residues: 266-618; Coverage: 57%)
Gene names: CG3041, Orc2
Sequence domains: Origin recognition complex subunit 2
Origin recognition complex subunit 3 Chain: C
Molecule details ›
Chain: C
Length: 676 amino acids
Theoretical weight: 77.65 KDa
Source organism: Drosophila melanogaster
Expression system: Trichoplusia ni
UniProt:
  • Canonical: Q7K2L1 (Residues: 46-721; Coverage: 94%)
Gene names: CG34315-RB, CG4088, DmORC3, DmOrc3, Dmel\CG4088, Dmel_CG4088, LAT, Lat, ORC, ORC3, Orc3, l(2)49Fd, l(2)vr6, lat, lat-RA, latheo, orc3, vr-6, vr6
Sequence domains:
Origin recognition complex subunit 5 Chain: E
Molecule details ›
Chain: E
Length: 460 amino acids
Theoretical weight: 52.18 KDa
Source organism: Drosophila melanogaster
Expression system: Trichoplusia ni
UniProt:
  • Canonical: Q24169 (Residues: 1-460; Coverage: 100%)
Gene names: CG7833, Orc5
Sequence domains:
Origin recognition complex subunit 1 Chain: A
Molecule details ›
Chain: A
Length: 393 amino acids
Theoretical weight: 43.91 KDa
Source organism: Drosophila melanogaster
Expression system: Trichoplusia ni
UniProt:
  • Canonical: O16810 (Residues: 532-924; Coverage: 43%)
Gene names: CG10667, Orc1
Sequence domains:
Origin recognition complex subunit 2 Chain: G
Molecule details ›
Chain: G
Length: 41 amino acids
Theoretical weight: 3.51 KDa
Source organism: Drosophila melanogaster
Expression system: Trichoplusia ni
Origin recognition complex subunit 6 Chain: F
Molecule details ›
Chain: F
Length: 72 amino acids
Theoretical weight: 8.2 KDa
Source organism: Drosophila melanogaster
Expression system: Trichoplusia ni
UniProt:
  • Canonical: Q9Y1B2 (Residues: 187-257; Coverage: 28%)
Gene names: CG1584, Orc6
Origin recognition complex subunit 4 Chain: D
Molecule details ›
Chain: D
Length: 459 amino acids
Theoretical weight: 52 KDa
Source organism: Drosophila melanogaster
Expression system: Trichoplusia ni
UniProt:
  • Canonical: Q9W102 (Residues: 1-459; Coverage: 100%)
Gene names: CG2917, DmORC4, Dmel\CG2917, Dmel_CG2917, ORC, ORC4, Orc4, dmOrc4, orc4, rDmORC
Sequence domains:

Ligands and Environments

2 bound ligands:
Ligand K 1 x K
Ligand CL 1 x CL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 23-ID-B
Spacegroup: I222
Unit cell:
a: 145.545Å b: 258.983Å c: 257.001Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.225 0.224 0.258
Expression system: Trichoplusia ni

Quick links

Citations

20 review citations

Chromosome Duplication in Saccharomyces cerevisiae.
Bell et al. (2016)
19 more

11 mentions without citation

Mechanisms and regulation of DNA replication initiation in eukaryotes.
Parker et al. (2017)
10 more