4xgb

X-ray diffraction
2.23Å resolution

Crystal Structure of E112A/H234A Mutant of Stationary Phase Survival Protein (SurE) from Salmonella typhimurium co-crystallized with AMP

Released:

Function and Biology Details

Reactions catalysed:
A 5'-ribonucleotide + H(2)O = a ribonucleoside + phosphate
A 3'-ribonucleotide + H(2)O = a ribonucleoside + phosphate
(Polyphosphate)(n) + H(2)O = (polyphosphate)(n-1) + phosphate
Biochemical function:
Biological process:
Cellular component:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
5'/3'-nucleotidase SurE Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 267 amino acids
Theoretical weight: 28.49 KDa
Source organism: Salmonella enterica subsp. enterica serovar Typhimurium str. LT2
Expression system: Escherichia coli
UniProt:
  • Canonical: P66881 (Residues: 1-253; Coverage: 100%)
Gene names: STM2927, surE
Sequence domains: Survival protein SurE
Structure domains: Survival protein SurE-like phosphatase/nucleotidase

Ligands and Environments

3 bound ligands:
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: BRUKER AXS MICROSTAR
Spacegroup: C2
Unit cell:
a: 161.42Å b: 96.52Å c: 94.51Å
α: 90° β: 99.34° γ: 90°
R-values:
R R work R free
0.211 0.208 0.262
Expression system: Escherichia coli