› 4xf9

X-ray diffraction
1.3Å resolution

cysteine dioxygenase variant - C93A at pH 8.0 in complex with homocysteine

Released:
DOI: 10.2210/pdb4xf9/pdb
PDB 4xf9 coloured by chain and viewed from the front.
PDB 4xf9 coloured by chain and viewed from the side.
PDB 4xf9 coloured by chain and viewed from the top.
Source organism: Rattus norvegicus
Primary publication:
Structure-Based Insights into the Role of the Cys-Tyr Crosslink and Inhibitor Recognition by Mammalian Cysteine Dioxygenase.
Driggers CM, Kean KM, Hirschberger LL, Cooley RB, Stipanuk MH, Karplus PA
J. Mol. Biol. 428 3999-4012 (2016)
PMID: 27477048

Function and Biology Details

Reaction catalysed: 
L-cysteine + O(2) = 3-sulfinoalanine
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Cysteine dioxygenase type 1 Chain: A
Molecule details ›
Chain: A
Length: 200 amino acids
Theoretical weight: 23.03 KDa
Source organism: Rattus norvegicus
Expression system: Escherichia coli
UniProt:
  • Canonical: P21816 (Residues: 1-200; Coverage: 100%)
Gene name: Cdo1
Sequence domains: Cysteine dioxygenase type I
Structure domains: Jelly Rolls

Ligands and Environments

2 bound ligands:
Ligand FE 1 x FE
Ligand HCS 1 x HCS
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 5.0.1
Spacegroup: P43212
Unit cell:
a: 57.6Å b: 57.6Å c: 122.4Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.16 0.157 0.186
Expression system: Escherichia coli

Quick links

Citations

3 review citations

Carbon-fluorine bond cleavage mediated by metalloenzymes.
Wang et al. (2020)
2 more

1 mention without citation

Structure-Based Insights into the Role of the Cys-Tyr Crosslink and Inhibitor Recognition by Mammalian Cysteine Dioxygenase.
Driggers et al. (2016)