4xeq

X-ray diffraction
1.7Å resolution

CRYSTAL STRUCTURE OF A TRAP PERIPLASMIC SOLUTE BINDING PROTEIN FROM DESULFOVIBRIO VULGARIS (Deval_0042, TARGET EFI-510114) BOUND TO COPURIFIED (R)-PANTOIC ACID

Released:
Source organism: Desulfovibrio vulgaris RCH1
Entry authors: Vetting MW, Al Obaidi NF, Toro R, Morisco LL, Benach J, Wasserman SR, Attonito JD, Scott Glenn A, Chamala S, Chowdhury S, Lafleur J, Love J, Seidel RD, Whalen KL, Gerlt JA, Almo SC, Enzyme Function Initiative (EFI)

Function and Biology Details

Structure analysis Details

Assembly composition:
monomeric (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
TRAP dicarboxylate transporter, DctP subunit Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 328 amino acids
Theoretical weight: 37.32 KDa
Source organism: Desulfovibrio vulgaris RCH1
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: A0A0E0SY65 (Residues: 37-341; Coverage: 100%)
Gene name: Deval_0042
Sequence domains: Bacterial extracellular solute-binding protein, family 7
Structure domains: Bacterial extracellular solute-binding protein, family 7

Ligands and Environments

1 bound ligand:
1 modified residue:

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 31-ID
Spacegroup: P21
Unit cell:
a: 83.75Å b: 73.6Å c: 100.21Å
α: 90° β: 90.05° γ: 90°
R-values:
R R work R free
0.164 0.157 0.185
Expression system: Escherichia coli BL21(DE3)